Reliability, robustness, systems biology, aging, stable magnetic isotopes, nuclear spin catalysis, free radicals, antioxidants, chemical nano-bionics.

1966 (June): graduated Taras Shevchenko State University in Kiev, Ukraine (former Soviet Union), Department of Physics. Master Degree in Physics (Diploma with Honor), topic: “EPR of gamma-irradiated amino-acids and proteins” (first diploma on biophysics in Ukraine, advisor Prof. Dmytro M. Grodzinsky).

1966-1968: research fellow in Department of Radiation Biology, Institute of Plant Physiology, Ukrainian Academy of Sciences, Kiev, Ukraine. 1967-1968: research fellow in laboratory of physical chemistry of biopolymers, Institute of Chemical Physics of USSR Academy of Sciences, Moscow.

1968 (Dec.) - 1971 (Nov.): pre-doctoral fellow (aspirant), Branch of Institute of Chemical Physics, Chernogolovka.

1971 (November): Ph. D. degree (Candidate of Physical and Mathematical Sciences – Chemical Physics), Institute of Chemical Physics, USSR Academy of Sciences, Moscow. Dissertation: "Studies of the electron-transport biological membranes by the method of the molecular probes", advisor: Prof. Lev A. Blumenfeld, opponents M.V. Volkenstein, A.P. Purmal). The first, in the world, studies of biological membranes with using of stable nitroxyl radicals and EPR (electron paramagnetic resonance) spectroscopy – the spin-probe method. Liquid state of lipid bilayers, thermo-dependent conformational transitions and ATP-dependent conformational transitions in the membrane proteins were first detected in these works.

1972 (January) – Present: work in Institute of Problems of Chemicals Physics (former Branch of Institute of Chemical Physics), Chernogolovka. In Department of Kinetics of Chemical and Biological Processes, headed by academician N. M. Emanuel, Dr. Koltover was engaged in the studies on the problems of free radicals, antioxidants and aging. In collaboration with Ukrainian colleagues (Institute of Gerontology of Academy of Medical Sciences of USSR, Kiev), it had been first manifested that one of the most effective antioxidants, 2,6-di-tert-butyl-4-methylphenol (called ionol or dibunol in Russian literature, butylated hydroxytoluene, BHT, in English- literature), acts on the organism’s endocrine system. Through the hormonal regulation system, this antioxidant prevents generation of anion-radicals of oxygen (“superoxide radicals”) as by-products of mitochondrial respiration. Contrary to the in vitro studies, the so-called antioxidants work in living systems, in vivo, not as the direct inhibitors of free-radical reactions but exert the preventive maintenance against “oxidative stress” via prevention of appearance of the free-radicals or via induction of expression of the antioxidant enzymes.

1988: Sci. D. degree (Doctor of Biological Sciences – Biological Physics). Dissertation topic: "Reliability of electron-transport membranes and the role of oxygen anion-radicals in aging". The systems reliability approach to the problem of aging was put forward. This approach is based on the simple general principles that (i) all biomolecular constructions are designed in keeping with the genetic programs in order to perform the programmed, preset functions; (ii) all of them operate with the limited reliability; (iii) the timely replacement or prophylaxis of unreliable functional elements, i.e. the metabolic turnover, is the main line of assuring the high systems reliability; and (iv) there is a finite number of critical elements which perform the supervisory functions over the preventive maintenance and these “supervisors” also operate with the limited, genetically preset, reliability. On this basis, of the theory of reliability, the universal features of aging, such as the exponential growth of mortality rate with time and the correlation of longevity with the species-specific resting metabolism are naturally explained. The stochastic malfunctions of the mitochondrial electron transport nanoreactors, which produce superoxide radicals, seem to be of first importance. The longevity of human brain could reach 250 years should the antioxidant enzyme defense against the free-radical failures be perfect. Thus, aging occurs as the consequence of the limited, genetically preset, reliability of the biomolecular constructions while the free-radical timer serves as the effective stochastic mechanism of realization of the program of aging. Besides, the systems reliability approach serves as heuristic methodology for development of novel preventive medicine.

In collaboration with Austrian colleagues, in order to elucidate the role of oxygen free radicals and mitochondria in aging, mitochondria isolated from hearts of rats of different age were exposed to metabolic conditions which are developed in cells and tissues during ischemia/reperfusion. It has been documented that even short-term anoxia/ischemia affects the electron transfer through redox-cycling ubisemiquinones. As consequence, the impaired mitochondria, especially the mitochondria isolated from old animals, become active generators of the potentially dangerous superoxide radicals.

In the eighties-nineties, after the atomic catastrophe in Chernobyl (Ukraine, 1986), much attention had been devoted to problems of radiation ecology, in part, the problem of radon. Radon and the radioactive products of its disintegration are the source of the main contribution to the radiation background of residential and industrial buildings. Not less than ten percent of the cases of lung cancer, regis­tered annually in the world, are caused by radon radiation. Therefore, the problem of radon is a focus of multidisciplinary radioecological programs in the United States, Japan, and Europe. Comprehensive studies of the danger of radon have also been started in Russia.

Then, in the nineties, Dr. Koltover as physicist and specialist in the magnetic-resonance spectroscopy headed the research projects on the novel class of chemical compounds, endohedral metallofullerenes. The runoff of the electron spin density from the central paramagnetic metal atom outside the carbon cluster was revealed. The “spin leakage” of the fullerene cage was testified by EPR, ENDOR and NMR methods in cooperation with the scientists in India and USA. Owing this effect, the paramagnetic endometallofullerenes hold much promise as the basic elements for realizing nanometer scale devices, amongst them – molecular magnets, superconductors with low critical temperatures and quantum dots.

Considerable recent attention has been focused on the magnetic isotope effects in living nature. All biomolecular devices are composed from atoms of chemical elements many of which have magnetic and nonmagnetic stable isotopes. The experiments with living cells enriched with different magnesium isotopes, magnetic ²⁵Mg or nonmagnetic ²⁴Mg or ²⁶Mg, had been, for the first time, performed. In experiments with yeast cells S. cerevisiae, in collaboration with Ukrainian colleagues and Petersburg Institute of Nuclear Physics (branch of the SRC “Kurchatov Institute”), it was  revealed that the rate constant of the post-radiation recovery of the irradiated cells is twice higher for the cells enriched with magnetic ²⁵Mg when compared to the cells enriched with the nonmagnetic isotope. The magnetic-isotope effects of ²⁵Mg were also detected in experiments with another commonly accepted cell model, bacteria E. coli. Furthermore, in experiments with the Ukrainian colleagues, Palladin Institute of Biochemistry in Kiev, it was revealed that the nuclear spin of magnetic ²⁵Mg improves efficiency of operation of muscle myosin, one of the most important enzymes of cell bioenergetics. Namely, ²⁵Mg essentially accelerates the enzymatic ATP hydrolysis, 2-2.5 times, by comparison to the spin-less ²⁴Mg or ²⁶Mg. The similar magnetic isotope effect, the nuclear spin catalysis, was detected in the experiments with the isotopes of zinc as the cofactor of the enzyme.

As invited scientist (visiting professor), Dr. Koltover worked in USA (National Lawrence Berkeley Laboratory, University of California, February, 1989, June, 2002; Department for Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, November-December, 1990; Department of Chemistry, Northwestern University, Evanston, July, 2001). Besides, he worked in Austria (Institute of Pharmacology and Toxicology, University of Veterinary Medicine, Vienna, July, 1991 – June, 1992); Germany (Max-Planck Institute for Demographic Research, Rostock, November, 2004, Institute for Physical Chemistry, Albert-Ludwig University, Freiburg, August, 2011); India (Indian Institute of Technology, Chennai, and Bhabha Atomic Center, Mumbai, January-February, 2002); Israel (Shamoon College of Engineering, Beer Sheva, December, 2011).

Plenary and oral presentations at international scientific meetings in Austria (1992, 1994), Canada (2001), China (2004, 2007, 2011), France (1996, 1998, 2003, 2005, 2009), Greece (2006), India (2002), Italy (1992, 2014), Israel (2010, 2016), Japan (1998), USA (1996, 2009, 2010), Ukraine and Russia.

Membership in professional societies and committees

Committee on Reliability of Biological Systems at the Scientific Council on the Problems of Biophysics, USSR Academy of Sciences, vice-chairman (1978-1992); Russian Biophysical Society, Russian Gerontology Society; international journal “Advances in Gerontology”, Saint-Petersburg, Russia (editor council); international journals “Aging and Longevity”, Kiev, Ukraine, (editor board), “Research in Medical & Engineering Sciences”, New York, USA, Crimson Publ (editor board), “Gerontology & Geriatrics Studies”, New York, USA, Crimson Publ (editor board); Russian Foundation for Basic Research (expert); Russian Scientific Foundation (expert). Never been in the Communistic Party of the Soviet Union.

Representative publications (selected from more than 300 papers):
Books

Koltover V.K., Skipa T.A. Free-radical redox timer, sirtuins and aging: from chemistry of free radicals to systems theory of reliability. In: Sirtuin Biology in Medicine. Targeting New Avenues of Care in Developments, ­Aging and Disease (ed. K. Maiese). ­London (UK), San Diego (CA, US), Cambridge (MA, US), Oxford (UK): Acad.  Press, Elsevier Inc.,  2021, pp. 213-224. ISBN: 978-0-12-814118-2).

Koltover V.K. Mathematical theory of reliability and biological robustness: Reliable systems from unreliable elements. In: Focus on Systems Theory Research (eds. M. Casanova, I. Opris). New York: Nova Science Publishers Inc., 2019, pp. 49-80. ISBN: 978-1-53614-561-8.

Koltover V.K., Labyntseva R.D., Kosterin S.O. Stable magnetic isotopes as modulators of ATPase activity of smooth muscle myosin. In: Myosin: Biosynthesis, Classes and Function (ed. D. Broadbent). New York: Nova Science Publishers Inc., 2018, pp. 135-158. ISBN: 978-1-53613-817-7.

Koltover V.K. Reliability and longevity of biological systems: The free-radical redox timer of aging. In: Redox Signaling and Biomarkers in Ageing. Healthy Ageing and Longevity (ed. U. Çakatay). Springer, Cham, 2022, vol. 15, Chapter 2, pp. 21-44. ISBN978-3-030-84965-8.

Koltover V.K., Skipa T.A. Free-radical redox timer, sirtuins and aging: From chemistry of free radicals to systems theory of reliability. In: Sirtuin Biology in Medicine. Targeting New Avenues of Care in Development, Aging, and Disease (ed. K. Maiese). Elsevier/Acad. Press, San Diego (USA), 2021, pp. 213-224. ISBN: 978-0-12-814118-2.

Koltover V.K. Mathematical theory of reliability and biological robustness: Reliable systems from unreliable elements. In: Focus on Systems Theory Research (eds. M.F. Casanova, I. Opris). Nova Science Publishers Inc., New York (USA), 2019, pp. 49-80. ISBN: 978-1-53614-561-8.

Koltover V.K., Labyntseva R.D., Kosterin S.O. Stable magnetic isotopes as modulators of ATPase activity of smooth muscle myosin. In: Myosin: Biosynthesis, Classes and Function (ed. D. Broadbent). Nova Science Publishers Inc., New York (USA), 2018, pp. 135-158. ISBN: 978-1-53613-817-7.

Koltover V.K. Antioxidant therapy of aging: from free radical chemistry to systems theory of reliability. In: Anti–Aging Drugs: from Basic Research to Clinical Practice (eds. D. Thurston, A. Vaiserman). Royal Society of Chemistry Publishing: Cambridge (UK), 2017, Chapter 8, pp. 183-204.

Koltover V.K. Nuclear spin effects in physics of living nature. In: Horizons in World Physics, Vol. 286 (ed. A. Reimer). New York: Nova Science Publishers Inc., 2015, pp. 65-78. ISBN: 978-1-63483-386-8.

Koltover V.K. Theory of reliability in systems biology: aging versus reliability. In: Recent Advances in Systems Biology Research (eds. A. Valente et al.). New York: Nova Science Publishers Inc., 2014, pp. 109-130. ISBN: 978-1-62948-736-6.

Koltover V.K., Korolev V.G., Kutlakhmedov Y.A. Antioxidant prophylaxis of radiation stress. In: Ionizing Radiation: Applications, Sources and Biological Effects. New York: Nova Science Publishers Inc., 2012, pp.117-128. ISBN: 978-1-62257-359-2.

Koltover V.K. Stable magnetic isotopes as a new trend in biomedicine. In: Biomedicine (ed. C. Lin). Rijeka: InTech-Europe, 2012, pp. 105-122. ISBN: 978-953-51-0352-3.

Koltover V.K. Paramagnetic endohedral fullerenes. In: Advances in Materials Science Research (ed. M. Wythers). New York: Nova Science Publishers Inc., 2011, Vol. 1, pp. 259-275. ISBN: 978-1-61728-109-9.

Koltover V.K. Endohedral fullerenes: from chemical physics to nanotechnology and  nanomedicine. In: Progress in Fullerene Research (ed. M. Lang). New York: Nova Science Publishers Inc., 2007, pp. 199-233.

Kutlakhmedov Y.A., Korogodin V.I., and Koltover V.K. Foundations of Radiation Ecology. Kiev (Ukraine): Vyshcha shkola, 2003. 320 P.-book (Ukraine).

Koltover V.K. Free radical theory of aging: view against the reliability theory. In: Free Radicals and Aging (eds. I. Emerit, B. Chance), Basel: Birkhauser, 1992, pp. 11-19.

Grodzinsky D.M., Vojtenko V.P., Kutlakhmedov Y.A., and Koltover V.K. Reliability and Aging of Biological Systems. Kiev (fSU): Naukova dumka, 1987. 172 P.-book (Russian).

Koltover V.K. Use of the spin-probe method in membrane biophysics. In: Biophysics, Vol. 4 – Methods used to study the structure of biological membranes (ed. Yu. A. Vladimirov), Boston: G.K. Hall and Co., 1977, pp. 10-68.

Koltover V.K. Application of spin probes to the study of various biological systems. In: G. Likhtenstein. Spin Labelling Methods in Molecular Biology. New York: Wiley & Sons, 1976, pp. 190-222.

Conference Proceedings

Koltover V.K. Radiative aspects in physics of liquid matter: Stable magnetic isotopes as new trend in anti-radiation defense. In: Modern Problems of the Physics of Liquid Systems – PLMMP 2018 (eds. L. Bulavin, L. Xu). Cham (Switzerland): Springer Nature, 2019, pp. 301-312. DOI https://doi.org/10.1007/978-3-030-21755-6_12.

Koltover V.K. Radiative Aspects in Physics of Liquid Matter: Stable Magnetic Isotopes as New Trend in Anti-radiation Defense. In: Modern Problems of the Physics of Liquid Systems – PLMMP 2018 (eds. L. Bulavin, L. Xu), Springer Proceedings in Physics, vol 223. Springer, Cham, 2019, pp. 301-312. ISBN 978-3-030-21755-6.

Koltover V.K. Mathematical theory of reliability and aging: Teaching comes from Kiev. In: Proceedings of the Second International Symposium on Stochastic Models in Reliability Engineering, Life Science and Operations Management (SMRLO’16), 2016, pp. 386-392, IEEE CPS (The Institute of Electrical and Electronics Engineers Inc., Conference Publishing Services), 978-1-4673-9941-8/16, DOI 10.1109/SMRLO.2016.65.

Koltover V.K. Nuclear spin catalysis: from molecular liquids to biomolecular nanoreactors. In: Physics of Liquid Matter: Modern Problems (eds. L. Bulavin, N. Lebovka). Springer Cham: Heidelberg, New York, Dordrecht, London, 2015, pp. 357-368. ISBN: 978-3-319-20874-9.

Koltover V.K. Reliability of nano devices and systems: Nuclear spins of stable magnetic isotopes as the reliability factors of biomolecular nanoreactors. In: Nanotechnology 2008, Vol. 1, Danville (CA, USA): Nano Science and Technology Institute, 2008, pp. 254-257.

Koltover V.K. Free radical theory of aging: Quantitative reliability aspects and antioxidant intervention. In: Vienna Aging Series, Vol.5: Vitality, Mortality and Aging (eds. A. Viidik, G. Hofecker). Vienna: Facultas, 1996, pp. 173-180.

Nohl H., Koltover V. An experimental approach to explain the age-related increase in oxygen radical generation during cell respiration. In: Vienna Aging Series, Vol. 4: Aspects of Aging and Disease (eds. D. Knook, G. Hofecker). Vienna: Facultas, 1994, pp. 55-57.

Peer reviewed journals

Koltover V.K. Nuclear spin catalytic effects as evidence for free-radical Intermediates in biochemical reactions driven by molecular motors. //Free Radic. Biol. Med. 2020, 159, Suppl. 1, 24-25.

Koltover V.K., Labyntseva R.D., Karandashev V.K. Magnetic-isotope effects of magnesium and zinc in the enzymatic ATP hydrolysis reactions driven by molecular motors. //Biophysics, 2020, 65, no. 3, 416-425.

Avdeeva L.V., Evstyukhina T.A., Koltover V.K., Korolev V.G., Kutlakhmedov Y.A.. Recovery of the yeast cells from radiation injuries with aid of the magnetic isotopes: A new trend in anti-radiation biomedicine. //Nucl. Physics Atom. Energy, 2019, 20, no. 3, 271-277.

Smirnova D.V., Koltover V.K., Nosenko S.V., Strizhova I.A., Ugarova N.N. Firefly luciferase bioluminescence as a tool for searching magnetic isotope effects in ATP-dependent enzyme reactions. //Moscow University Chemistry Bull., 2018, 73, no. 4, 158-165.

Koltover V.K. Nuclear spin catalysis in biochemical physics. // Russ. Chem. Bull., Inter. Edition, 2021, 70, no. 9, 1633-1639.

Koltover V.K., Labyntseva R.D., Karandashev V.K.  Magnetic-isotope effects of magnesium and zinc in the enzymatic ATP hydrolysis reactions driven by molecular motors. // Biophysics (Engl. Translation), 2020, 65, no. 3, 416-425.

Avdeeva L.V., Evstyukhina T.A., Koltover V.K., Korolev V.G., Kutlakhmedov Y.A..Recovery of the yeast cells from radiation injuries with aid of the magnetic isotopes:A new trend in anti-radiation biomedicine. // Nuclear  Physics and Atomic Energy, 2019, 20, no. 3, 271-277.

Skipa T.A., Koltover V.K. Fullerene trend in biomedicine: expectations and reality. // Res. Med. Eng. Sci., 2019, 8, no. 3, 877-879.

Smirnova D.V., Koltover V.K., Nosenko S.V., Strizhova I.A., Ugarova N.N. Firefly luciferase bioluminescence as a tool for searching magnetic isotope effects in ATP-dependent enzyme reactions. // Moscow University  Chemistry Bull., 2018, 73, no. 4, 158-165.

Koltover V.K. Antioxidant biomedicine: from chemistry of free-radicals to reliability of biological systems. // Res. Med. Eng. Sci., 2018, 3, no. 3, 1-6

Koltover V.K. Mathematical theory of reliability and aging: a little bit of history and the state of art. // Gerontology and Geriatrics Studies. 2018, 4, no. 2, 1-2.

Koltover V.K. Nuclear spin catalysis: from physics of liquid matter to medical physics. //J. Mol. Liquids, 2017, 235, 44-48.

Koltover V.K. Free radical timer of aging: from chemistry of free radicals to systems theory of reliability. //Current Aging Science, 2017, 10, no. 1, 12-17.

Koltover V.K. Redox timer of aging: from free radical chemistry to systems theory of reliability. //Free Radic. Biol. Med. 2017, 112, Suppl. 1, 53-54.

Avdeeva L.V., Koltover V.K. Nuclear spin catalysis in living nature. //Moscow University Chemistry Bulletin. 2016, 57, no. 3, 160-166.

Koltover V.K., Labyntseva R.D., Karandashev V.K., Kosterin S.O. Magnetic isotope of magnesium accelerates ATP hydrolysis catalyzed by myosin. //Biophysics. 2016, 61, no. 2, 200-206.

Gorban E.N., Koltover V.K., Sanina N.A., Bezrukov V.V., Aldoshin S.M. Thionitrosyl binuclear iron complexes as new class of agents for anti-aging therapy. //Free Radic. Biol. Med. 2015, 87, Suppl. 1, S79.

Koltover V.K. Stable magnetic isotopes: from spin chemistry to biomedicine. //Russian Chemical Bull.. 2014, 63, no. 5, 1029-1035.

Koltover V.K. Nuclear spin catalysis in nanoreactors of living cells. //Biophysics. 2013, 58, no. 2, 187-192.

Koltover V.K., Shevchenko U.G., Avdeeva L.V., Royba E.A., Berdinsky V.L., Kudryashova E.A. Magnetic isotope effect of magnesium in the living cell. //Doklady Biochem. Biophys. 2012. 442, no. 1-2, 12-14 (communicated by academician A.E. Shilov).

Koltover V., Kutlakhmedov Y., Grodzinsky D. The living cell perceives the difference between magnetic and nonmagnetic isotopes of magnesium. //J. Mol. Biol. Cell, 2011, 22, 441.

Kol’tover V.K. Reliability of electron-transport membranes and the role of oxygen radical anions in aging: stochastic modulation of the genetic program. //Biophysics. 2011, 56, no. 1, 125–128.

Koltover V.K. Antioxidant biomedicine: from free radical chemistry to systems biology mechanisms. //Russian Chemical Bull. 2010, 59, no. 1, 37-42.

Koltover V.K. Bioantioxidants: The systems reliability standpoint. //Toxicology and Industrial Health. 2009, 25, no. 4-5, 295-299.

Koltover V.K. Endofullerenes: from chemical physics to base elements for nanotechnologies and nanomedicine. //Mendeleev Communications (Ross. Khim. Zhurn., Engl. Translation). 2009, 53, no. 2, 79-85.

Goncharova N.D, Shmaliy A.V, Bogatyrenko T.N., Koltover V.K. Correlation between the activity of antioxidant enzymes and the circadian rhythms of corticosteroids in Macaca mulatta monkeys of different age. //Experimental Gerontol. 2006, 41, no. 8, 778-783.

Koltover V.K. Nanoparticles of endohedral metallofullerenes. //J. Mol. Liquids. 2006, 127, 139-141.

Koltover V.K., Logan J.W., Heise H., Bubnov V.P., Estrin Y.I., Kareev I.I., Lodygina V.P., Pines A. Diamagnetic clusters of paramagnetic endometallofullerenes: A solid state MAS NMR study. //J. Phys. Chem. B. 2004, 108, no. 33, 12450-12455.

Koltover V.K. Spin-leakage of the fullerene shell of endometallofullerenes: EPR, ENDOR and NMR evidences. //Carbon. 2004, 42, no. 5/6, 1179-1183.

Nath S., Pal H., Sapre A.V., Bubnov V.P., Estrin Y.I., Parnyuk T.A., Koltover V.K. Aggregation of endometallofullerene Y@C₈₂ in polar solvents. //Fullerenes, Nanotubes, and Carbon Nanostructures. 2004, 12, no. 1, 53-57.

Koltover V.P., Bubnov V.P., Estrin Y.I., Lodygina V.P., Davydov R.M., Subramoni M., Manoharan P. T. Spin-transfer complexes of endohedral metallofullerenes: ENDOR and NMR evidences. //Phys. Chem. Chem. Phys. 2003, 5, no. 13, 2774-2777.

Bubnov V.P., Laukhina E.E., Kareev I.E., Koltover V.K., Prokhorova T.G., Yagubskii E.B., Kozmin Y.P. Endohedral metallofullerenes: A convenient gram-scale preparation. //Chem. Mater. 2002, 14, no. 3, 1004-1008.

Koltover V.K., Parnyuk T.A., Bubnov V.P., Davydov R.M. Permeability of the fullerene cage of endometallofullerene for electron spin density. //Doklady Physics. 2002, 384, no. 1-3, 329-331 (communicated by academician Yu.A. Ossipyan).

Koltover V.K., Parnyuk T.A., Bubnov V.P., Laukhina E.E., Estrin Y.I., Yagubskii E.B. Stability and mobility of the endohedral metallofullerene La@C₈₂ in polycarbonate polymer films. //Phys. Sol. State. 2002, 44, no. 3, 529-530.

Koltover V.K. Historical predispositions and evolution of the free-radical theory of aging). //Problems of Aging and Longevity (Kiev, Ukraine). 2000, 9, no. 1, 78-89 (Russian).

Koltover V.K. Free radical theory of aging: today and tomorrow. //Advances in Gerontology. 1998, 2, 37-42 (Russian).

Koltover V.K. Reliability concept as a trend in biophysics of aging. //J. Theor. Biol. 1997, 184, no. 2, 157-163.

Koltover V.K. Radon radiation: sources, doses, and biological effects. //Herald of the Russian Academy of Sciences. 1996, 66, no. 2, 26-31.

Koltover V.K. The anti-hypoxic action of antioxidant BHT mediated via nitric-oxide: A study of EPR signals in tissues of rats of different ages. //AGE (J. Amer. Aging. Ass.). 1995, 18, № 3, 85-89.

Nohl H., Koltover V., Stolze K. Ischemia/reperfusion impairs mitochondrial energy conservation and triggers O₂^ˉ release as a by-product of respiration. //Free Radic. Res. Communs. 1993, 18, № 3, 127-137.

Frolkis V.V., Gorban E.N. Koltover V.K. Effects of antioxidant butylated hydroxytoluene (BHT) on hormonal regulation and ESR signals in adult and old rats. //AGE (J. Amer. Aging. Ass.) 1990, 13, № 1, 5-8.

Kapitanov A.B., Aksenov M.Y., Tatishchev O.S., Koltover V.K. Cell-culture of Acholeplasma laidlawii as an object for the investihation of the age-variation of biological membranes. //Doklady Biophysics. 1985, 281, no. 1, 186-189 (communicated by academician N.M. Emanuel).

Frolkis V.V., Gorban E.N., Koltover V.K. Effect of butylated hydroxytoluene antioxidant (dibunol) on hormonal regulation in rats of different age. //Doklady Biophysics. 1985, 284, no. 2, 210-213 (communicated by academician O.G. Gazenko).

Gus'kova R.I., Ivanov I.I., Koltover V.K., Akhobadze V.V., Rubin A.B. Permeability of bilayer lipid membranes for superoxide (O₂^─). //Biochim. Biophys. Acta. 1984, 778, 579-585.

Kochetkov V.V., Likhtenstein G.I., Koltover V.K., Knox P.P., Kononenko A.A., Grishanova P.G., Rubin A.B. Intramolecular dynamics and electron transfer in photosynthetic reaction centers - Study by the method of spin labels. //Bulletin of the USSR Academy of Sciences. 1984, no. 4, 572-578.

Milyutin A.A., Belyayeva E.I., Bulanova K.Ya., Kirilyuk A.P., Lyskova T.I., Okun I.M., Aksentsev S.L., Konev S.V., Koltover V.K. Structural and functional changes of rat brain synaptic membranes in aging. //Biofizika (Moscow). 1984, 29, no. 4, 640-642.

Koltover V.K. Theory of reliability, superoxide radicals and aging. //Achievements in the Life Sciences. 1983, 96, no. 4, 85-100.

Koltover V.K. Reliability of enzyme systems and molecular mechanisms of ageing. //Biofizika (Moscow). 1982, 27, no. 4, 635-639.

Koltover V.K. Reliability of enzymatic protection of a cell against superoxide radicals and the aging. //Doklady Biophysics (DOKL AKAD NAUK SSSR). 1981, 256, no. 1, 3-5 (communicated by academician N.N. Semenov).

Koltover V.K. Kutlakhmedov Y.A., Afanaseva E.L. Recovery of cells from radiation-induced damages in the presence of antioxidants and the reliability of biological systems. //Doklady Biophysics. 1980, 254, 159-161 (communicated by academician N.M. Emanuel).

Gus’kova R.A., Vilenchik M.M., Koltover V.K. Role of free superoxide radicals in the aging of biological objects. //Biofizika (Moscow). 1980, 25, no. 1, 102-105.

Koltover V.K. Application of the spin-probe method in studies of biological membranes. //Russian Advances in Biol. Chemistry. 1974, 15, 232-254.

Koltover V.K., Blumenfeld, L.A. Thermo-conformational transitions in electronic carrying biological membranes. //Biofizika (Moscow). 1973, 18, no. 5, 827-833.

Bluimenfeld L.A., Koltover V.K. Energy transformation and conformational transitions in mitochondrial membranes as relaxation processes. //Mol. Biol. 1972, 6, No. 1,130-133.

Koltover V.K., Reichman L.M., Yasajtis A.A., Blumenfeld L.A. A study of spin-probe solubility in mitochondrial membranes correlated with ATP-dependent conformation changes. //Biochim. Biophys. Acta. 1971, 234, 296-310.

Goldfeld M.G., Koltover V.K., Rozantsev E.G., Suskina V.I. Untersuchung der Strukturbildung in Kolloidsystemen mit der Paramagnetsonde. //Kolloid.-Z. und Z. Polymere. 1971, 243, no. 1, 62-66.

Koltover V.K., Goldfeld M.G., Hendel L.Y., Rozantsev E.G. Conformation transition in biological membrane studied by the spin label method. //Biochem. Biophys. Res. Communs. 1968, 32, 421-425.

Koltover V.K., Kutlakhmedov Yu.A., Sukhorukov B.I. Using of the paramagnetic probe method for studies of membranes of cellular organelles. //Transactions of the USSR Academy of Sciences (DOKL AKAD NAUK SSSR). 1968, 181, no.3, 730-732 (communicated by academician G.M. Frank).