Biochemistry, cell biology, molecular biology, proteomics, mass spectrometric analysis, oncology, cardiology.

Akentieva N.P., Doctor of Biological Sciences - a well-known scientist in the field of studying the molecular mechanisms of action of dinitrosyl iron complexes (DNICs), researching the therapeutic potential of DNICs for the treatment of cardiovascular diseases. The scope of her scientific activity is also the identification and characterization of biomarkers for the early diagnosis of cancer. Design, synthesis and study of RHAMM-targeted peptides for the diagnosis and therapy of cancer. Development of nanoparticles for targeted drug delivery (antitumor and cardiological).

Molecular targets of dinitrosyl iron complexes (DNICs) have been identified for the first time. It has been shown that DNICs are effective inhibitors of myeloperoxidase, matrix metalloproteinase, renin, biomarkers of cardiovascular diseases. The effect of DNICs on metabolic processes in cells (fibroblasts, cardiomyocytes) was studied. It has been shown that DNICs have therapeutic potential for the treatment of cardiac diseases and cardiac complications of cancer patients during chemotherapy.

For the first time, a design and synthesis of RHAMM-target peptides was carried out, and their diagnostic and therapeutic potential for the treatment of oncological diseases (breast cancer, prostate cancer, ovarian cancer) was studied. Nanoparticles have been developed for targeted delivery of anticancer drugs to cancer cells. The results of the study have expanded the understanding of the role of the RHAMM receptor in the development and progression of cancer.

The molecular mechanism of occurrence and development of periodontal disease has been studied. For the first time, adhesive proteins of the P.intermedia 17 bacterium, which is responsible for the development of periodontal disease, have been identified. These results have made a significant contribution to the understanding of the occurrence and development of periodontal disease.

The primary structure and post-translational modifications of the α₁ subunit of the GABA_A receptor have been studied for the first time. These results have expanded fundamental knowledge about the regulation of receptor activity and modulation of the activity of GABA_A receptors in the brain for the treatment of neurological diseases.

For the first time, a colchicine-binding site has been identified in tubulin, a key protein responsible for cell proliferation.

As a result of a joint study with the pharmaceutical company TROPHOS (France, 2003-2005), the drug TRO19622 (4-cholesten-3-one, oxime) was developed, the mechanism of action of TRO19622, a new compound for the treatment of multiple amyotrophic sclerosis, was studied. The results of the study are published in an article that is included in the International Encyclopedia. TRO19622 has been patented and commercialized for the treatment of multiple sclerosis.

It has been shown for the first time that the DRAG enzyme is able to form a complex with membrane proteins in the chromatophores of the bacterium Rhodospirillum rubrum. These results have contributed to the understanding of protein-protein interactions in bacteria.

The mechanism of C-H bond activation in methane and other alkanes has been studied for the first time, chemical models of anaerobic oxidation of hydrocarbons by methane-forming bacteria are presented. These results have created a new understanding that methanogenesis is reversible.

The membrane-bound enzyme methane monooxygenase from Methylococcus capsulatus (strain M) was isolated and purified for the first time. New fundamental knowledge about the physicochemical properties of methane monooxygenase was obtained, two-dimensional crystals of the enzyme were obtained for the first time, the quaternary structure and active center of methane monooxygenase were studied.

The structure, functional groups of the active site, and kinetic characteristics of glutamine synthetase from chloroplasts of pea leaves were studied for the first time. These results have deepened our understanding of the mechanism of action of glutamine synthetase, a key enzyme in ammonium assimilation.

Scientific awards, participation in scientific societies, editorial boards

Honorary diploma of the FASO RF for impeccable work and high achievements in professional activities, January 12, 2017, No. 5p.

Diploma of Ivanovo State University for the management of scientific activities of students, 2017.

Diploma for the best oral presentation at the 5th Interdisciplinary Scientific Forum with international participation "New materials and advanced technologies", October 31-November 1, 2019, Moscow, Russia.

Grant from the Russian Fund "Dynasty", October 22, 2013, Moscow, Russia.

Akentieva N.P. included as a Biographical Candidate in the “Who's Who” of World Renowned Professionals, Madison 2007-2008.

Postdoctoral Fellowship at Stockholm University, Sweden, 1999-2001.

Acknowledgment for active participation as a reviewer of student papers at the student conference “First Annual Data and Dine, the Pennsylvania State University, College of Medicine and the Milton S. Hershey Medical Center, February 2002, Hershey, PA, USA.

Gratitude from the Minister of Education and Science of the Russian Federation for professionalism and outstanding contribution to the mega grants competition in 2011.

Gratitude from the Ministry of Education and Science of the Russian Federation for professionalism and outstanding contribution to the mega grants competition in 2013.

Gratitude from the Ministry of Education and Science of the Russian Federation for professionalism and outstanding contribution to the mega grants competition in 2014.

Acknowledgment from the Russian Science Foundation of the Russian Federation for professionalism and outstanding contribution to the grant competition in 2015.

Member of the International Association of Russian-Speaking Scientists (RASA), since 2010.

Member of Ru SciTech Society, 2014-2018, Russia.

Member of American Cell Biology Association, USA, 2010-2012.

Member of Neuroscience Society, 2005-2007, USA.

Member of the National Postdoctoral Association, 2005-2007, USA.

Member of the Postdoctoral Executive Committee of Washington University in Saint Louis, Head of the Postdoc Committee and Member of the Career Committee, 2005-2007, USA

          2014-present - RSF expert (Russian Science Foundation).

2014-present - expert of MEGA-grants of the Ministry of Education and Science of the Russian Federation.

2022 - to the present - expert of the project office of the state program of the Russian Federation "Scientific and technological development of the Russian Federation".

2011-2013 International expert of the Euro-Asia Foundation of International Scientific MEGA Grants of the Ministry of Education and Science of the Russian Federation.

Editor of the international journal "Journal of Solid Tumors", 2016-2018, Toronto, Canada.

Editor of the international journal "Journal of Applied and Theoretical Physics Research", 2016-2018, Verizona Publisher, London, Ontario, Canada.

Editor of the international journal "Journal of Pharmacology and Toxicology", 2018, USA.

Editor of the international journal "Journal of Carcinogenesis and Mutagenesis", 2018, USA.

Editor of the international journal "Journal of Current Pharmaceutical Design" (Web of Science), 2018, USA.

Editor of the international journal "Journal of Annals Breast Cancer", 2016-2018, USA.

Patents:

1. Naor D., Akentieva N.P., Turley E. Rhamm-derived peptide for treatment of diabetes. // International Patent 12459. June, 2013. 40 Yissum Research Development company of the Hebrew University of Jerusalim, Israel.

2. Evdokimov Yu.M., Akent'eva N.P., Gapeev A.A., Sulimenko V.A., Russkikh D.V., Meshcheryakov A.V. Patent No. 2021137314/04 (078503). A device for obtaining graphene. December 16, 2021, Russia.

• Natalia Akentieva. Nano-LC-FTMS Analysis of the Primary Structure and Post-translational Modifications of the α1 Subunit of the GABAA Receptor. // Biointerface Research in Applied Chemistry, 2022,   Volume 13, Issue 4, 2023, 363  P.1-18.  Web of Science,  Q3, IF=2.4.  https://doi.org/10.33263/BRIAC134.363  

• Natalia Akentieva, Artur Gizatullin , Natalia Saninа, Natalia Shkondina , Kseniya Abramova , Vladimir Tikhonov, Stanislav Shram,  Sergey Aldoshin  and Jana Nieder. Intracytoplasmic trafficking of nanoparticles based on hyaluronic acid and chitosan for targeted delivery of anticancer drugs. //Biointerface Research in Applied Chemistry, 2022,   Volume 13, Issue 4, 2023, 344.  P.1-16.  Web of Science,  Q3, IF=2.4. https://doi.org/10.33263/BRIAC134.344  

• Natalia Akentieva, Natalia Sanina, Artur Gizatullin, Natalia Shkondina ,  Anna Andreeva, Stanislav Shram , Sergei Aldoshin. Influence of Dinitrosyl Iron Complexes on the Activity of Enzymes, Indicators of Cardiovascular Diseases. //Biointerface Research in Applied Chemistry, 2022, Volume 13, Issue 2, 2023, 198, P.1-17. https://doi.org/10.33263/BRIAC132.198.

• Akentieva N.P., Shushanov S.S., Gizatullin A.R., Prikhodchenko T.R., Shkondina N.I., D’agaro E. The effect of plant extracts Kalanchoe daigremontiana and Aloe arborescens on the metabolism of human multiple myeloma cells. // Biointerface Res. Appl. Chem. – 2021. – Vol. 11(5). – PP. 13171 – 13186. https://doi.org/10.33263/BRIAC115.1317113186 , Web of Science, IF=1.68.

• Akentieva N.P., Topunov A.F. RHAMM-target peptides inhibit proliferation and viability of cancer cells. // Biointerface Res. Appl. Chem. – 2021. – Vol. 11(4). - PP. 12252 -12266. https://doi.org/10.33263/BRIAC114.1225212266 , Web of Science, IF=1.68.

• Natalia P. Akentieva, Alexey F. Topunov. RHAMM-Target Peptides as Molecular Imaging Probes for the Imaging of RHAMM-Expressing Cancer Cells. // Biointerface Res. Appl. Chemistry. – 2021(2022). - Vol. 12(1). - PP. 2-24. https://doi.org/10.33263/BRIAC121.002024 , Web of Science, IF=1.98.

• D’agaro E., Franco Rosa, Natalia P. Akentieva. New technology tools and life cycle analysis (LCA) applied to a sustainable Livestock Production. // Review. EuroBiotech J. – 2021. - Vol. 5(3). - PP. 130-141. https://doi.org/10.2478/ebtj-2021-0022, Web of Science.

• Akentieva N.P., Gizatullin A.R., Sanina N.A. ; Dremova N.N., Torbov V.I. ; Shkondina N.I., Zhelev N., Aldoshin S.M. Fabrication of chitosan-hyaluronic acid nanoparticles and encapsulation into nanoparticles of dinitrosyl iron complexes as potential cardiological drugs. Nanomedicine journal, Vol 7, # 3, PP. 199-210. Web of Science, DOI: 10.22038/nmj.2020.07.0004. Web of Science.

• N. P. Akentieva, N. A. Sanina, A. R. Gizatullin, N. I. Shkondina, T. R. Prikhodchenko, S.I. Shram, N. Zhelev and S. M. Aldoshin. Cytoprotective Effects of Dinitrosyl Iron Complexes on Viability of Human Fibroblasts and Cardiomyocytes. Frontiers in Pharmacology (Web of Science, Q1, IF=4.465), 2019, volume10, Article 1277, PP. 1-21. Doi:10.3389/fphar.2019.01277

• Akentieva, N. Posttranslational modification of dinitrogenase reductase in Rhodospirillum rubrum treated with fluoroacetate. // World  J. Microbiol. Biotechnol. -2018. - Vol. 34.- PP. 184-194. Web of Science,  https://doi.org/10.1007/s11274-018-2564-y/ IF=2.4, Q3.

• Akentieva Natalia. Mass Spectrometry Identification of Outer Membrane Proteins of Prevotella intermedia 17, Interacting with Host Cells. // Acta Scientific Cancer Biology, 2018, V. 2 (3),  PР. 02-13.

• Natalia Akentieva. RHAMM-target peptides inhibit invasion of breast cancer cells. // EuroBiotechnology J., 2017; vol. 1, p. 138-148. DOI: 10.24190/ISSN2564-615X/2017/02.05. Web of Science, IF-3.34.

• Esguerra KV, Tolg C, Akentieva N, Price M, Cho CF, Lewis JD, McCarthy JB, Turley EA, Luyt LG. Identification, design and synthesis of tubulin-derived peptides as novel hyaluronan mimetic ligands for the receptor for hyaluronan-mediated motility (RHAMM/HMMR). // Integrative Biology. (Camb). 2015, 7(12):1547-60. doi: 10.1039/c5ib00222b. (IF-4.455), Q2. Web of Science.

• Rizzardi A.E., Vogel R.I., Koopmeiners J.S., Forster C.L., Marston L.O., Rosener N.K., Akentieva N., Price M.A., Metzger G.J., Warlick C.A., Henriksen J.C., Turley E.A., McCarthy J.B., Schmechel S.C. Elevated hyaluronan and hyaluronan-mediated motility receptor are associated with biochemical failure in patients with intermediate-grade prostate tumors. // Cancer. 2014, Vol. 120 (12): 1800-9. (IF-6.537), Q1. Web of Science.

• Veiseh, M., Breadner D., Ma J, Akentieva, N. P., Savani R.C., Harrison R., Mikilus D., Collis L., Gustafson S., Lee T.Y.,  Koropatnick, J., Luyt L.G., Bissell, M. J., Turley, E. Imaging of homeostatic, neoplastic, and injured tissues by HA-base probes. // Biomacromolecules, Vol. 13 (1), p.12-22, 2012 (IF- 5.941), Q1, Web of Science.

• Tolg C., Telmer P., Ma J., Akentieva N.P., Luyt L.G., Turley E. A Rhamm mimetic peptide blocks hyaluronan fragment signaling and promotes skin excisional wound repair by reducing inflammation. //American Journal of pathology, Vol. 181 (4), 1250-70, 2012 (IF- 4.890), Q1, Web of Science.

• Chen Z., Chen LH, Akentieva N., Lichti CF, Darbandi R., Hastings R., Lichti Ch. F., Gross J., Covey D. F., Reichert D. E., Townsend R. , Alex S. Evers. A neurosteroid analogue photolabeling reagent labels the colchicines-binding site on tubulin: a mass spectrometric analysis.// Electrophoresis, 2012, 33 (4): 666-674. (IF-3. 303), Q2, Web of Science.

• Bordet T., Buisson B., Michaud M., Steidl E., Akentieva N., Evers A., Massaad C., Schumacher M., Ostuni M. A., Lacapère J.-J., Galea P., Drouot C., Maux D., Henderson C. E. & Pruss R. Identification and Characterization of TRO19622 (4-Cholesten-3-one, Oxime), a novel compound for the treatment Amyotrophic Lateral Sclerosis // Journal of Pharmacology and Experimental Therapeutics, 2007, 322 (2), 709-720. (IF-3.828), Q1, Web of Science.

Monographs

1. 1. N.P. Akent'eva, A.F. Topunov. The role of peptides in theranostics of oncological diseases. // M.: RAN, 2021. - 172 pages. ISBN 978-5-907366-48-0.

   2. Akent'eva N.P., Sanina N.A., Gizatullin A.R. Iron dinitrosyl complexes (nitric oxide donors) are promising drugs for the treatment of cardiovascular diseases. // M.: RAN, 2023. - 210 pages.